michaelis–menten enzyme kinetics analyses Search Results


recombinant human nep enzyme  (R&D Systems)
94
R&D Systems recombinant human nep enzyme
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Recombinant Human Nep Enzyme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human nep enzyme/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant human nep enzyme - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
prism version 6.00 windows  (GraphPad Software Inc)
90
GraphPad Software Inc prism version 6.00 windows
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Prism Version 6.00 Windows, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prism version 6.00 windows/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
prism version 6.00 windows - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
graphpad prism 10  (GraphPad Software Inc)
90
GraphPad Software Inc graphpad prism 10
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Graphpad Prism 10, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/graphpad prism 10/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
graphpad prism 10 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis-menten and lineweaver-burk functions of graphpad prism 5.0  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis-menten and lineweaver-burk functions of graphpad prism 5.0
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis Menten And Lineweaver Burk Functions Of Graphpad Prism 5.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis-menten and lineweaver-burk functions of graphpad prism 5.0/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis-menten and lineweaver-burk functions of graphpad prism 5.0 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
plotting and curvefitting tools  (MathWorks Inc)
90
MathWorks Inc plotting and curvefitting tools
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Plotting And Curvefitting Tools, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plotting and curvefitting tools/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
plotting and curvefitting tools - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis–menten equation  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis–menten equation
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis–Menten Equation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis–menten equation/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis–menten equation - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis-menten enzyme kinetics  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis-menten enzyme kinetics
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis Menten Enzyme Kinetics, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis-menten enzyme kinetics/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis-menten enzyme kinetics - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis-menten nonlinear regression model  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis-menten nonlinear regression model
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis Menten Nonlinear Regression Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis-menten nonlinear regression model/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis-menten nonlinear regression model - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
sigma plot 14.0 software  (SYSTAT)
90
SYSTAT sigma plot 14.0 software
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Sigma Plot 14.0 Software, supplied by SYSTAT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigma plot 14.0 software/product/SYSTAT
Average 90 stars, based on 1 article reviews
sigma plot 14.0 software - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis-menten model in graphpad prism v.6.05  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis-menten model in graphpad prism v.6.05
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis Menten Model In Graphpad Prism V.6.05, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis-menten model in graphpad prism v.6.05/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis-menten model in graphpad prism v.6.05 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
plot 12  (Millipore)
90
Millipore plot 12
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Plot 12, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plot 12/product/Millipore
Average 90 stars, based on 1 article reviews
plot 12 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
michaelis-menten enzyme kinetic s28 model  (GraphPad Software Inc)
90
GraphPad Software Inc michaelis-menten enzyme kinetic s28 model
Determination of the effect of pre-incubation of RSIY-11 with <t>NEP</t> on inhibition. Fifty microliters of <t>recombinant</t> human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference
Michaelis Menten Enzyme Kinetic S28 Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/michaelis-menten enzyme kinetic s28 model/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
michaelis-menten enzyme kinetic s28 model - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Determination of the effect of pre-incubation of RSIY-11 with NEP on inhibition. Fifty microliters of recombinant human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Identification and characterization of RSIY-11, a novel seminal peptide derived from semenogelin-1, which acts as a neutral endopeptidase inhibitor modulating sperm motility

doi: 10.1007/s10815-019-01524-8

Figure Lengend Snippet: Determination of the effect of pre-incubation of RSIY-11 with NEP on inhibition. Fifty microliters of recombinant human NEP enzyme (0.1 μg/ml) and 50 μl of RSIY-11 (8.2 μM) were incubated for 0, 3, or 24 h at 37 °C prior to the addition of 50 μl of fluorogenic NEP substrate to determine enzymatic activity, and then, activity was measured every minute over 15 min. A control was performed by incubating NEP for the indicated time without RSIY-11, which was used as the 100% for each time point. Assays were performed in triplicate and averaged. Enzymatic activity was expressed as a percentage of the activity of the control. Error bars represent standard deviation. Asterisks (*) = statistically different from the control, *p value < 0.05; **p value < 0.01. NS no significant difference

Article Snippet: Michaelis-Menten Constant A fluorometric assay was performed using recombinant human NEP enzyme at 0.1 μg/ml (R&D Systems, Minneapolis, USA) and fluorogenic NEP peptide substrate ((7-methoxycoumarin-4-yl)-acetyl-R-P-P-G-F-S-A-F-K-(2, 4-dinitrophenyl)-OH, R&D Systems).

Techniques: Incubation, Inhibition, Recombinant, Activity Assay, Standard Deviation